{"id":258,"date":"2021-01-28T22:45:53","date_gmt":"2021-01-28T14:45:53","guid":{"rendered":"http:\/\/www.wuchenshebei.com\/?p=258"},"modified":"2021-03-13T18:12:18","modified_gmt":"2021-03-13T10:12:18","slug":"%e4%bc%a0%e9%80%92%e7%aa%97%e5%9c%a8pcr%e5%ae%9e%e9%aa%8c%e5%ae%a4%e8%ae%be%e8%ae%a1%e8%a6%81%e6%b1%82%e3%80%90%e8%a7%84%e8%8c%83%e3%80%91","status":"publish","type":"post","link":"http:\/\/www.wuchenshebei.com\/258.html","title":{"rendered":"PCR\u5b9e\u9a8c\u5ba4\u4f20\u9012\u7a97\u8bbe\u8ba1\u8981\u6c42\u3010\u89c4\u8303\u3011"},"content":{"rendered":"

\u6ce8\uff1a\u7531\u4e8e2020\u5e74\u7a81\u53d1\u65b0\u51a0\uff0c\u4f20\u9012\u7a97\u5c31\u51fa\u73b0\u4e86\u4f9b\u4e0d\u5e94\u6c42\u7684\u5c40\u9762\uff0c\u76ee\u524d\u4f20\u9012\u7a97\u5e94\u7528\u4e8e\u5404\u5927\u533b\u9662\u3001PCR\u5b9e\u9a8c\u5ba4\u3001\u75c5\u623f\u7b49\u5c0f\u7269\u54c1\u7684\u4f20\u9012\uff0c\u76ee\u7684\u662f\u4e3a\u4e86\u9632\u6b62\u7a7a\u6c14\u5bf9\u6d41\u3001\u907f\u514d\u4ea4\u53c9\u611f\u67d3\uff0c\u540c\u65f6\u4f20\u9012\u7a97\u5185\u5b89\u88c5\u6709\u7d2b\u5916\u7ebf\u6740\u83cc\u706f\uff0c\u8d77\u5230\u5f88\u597d\u7684\u706d\u83cc\u4f5c\u7528\u3002<\/p>\n

\"PCR\u5b9e\u9a8c\u5ba4\u4f20\u9012\u7a97\u8bbe\u8ba1\u8981\u6c42\u3010\u89c4\u8303\u3011\"

\u5185\u5d4c\u5f0f\u4f20\u9012\u7a97<\/p><\/div>\n

1 \u3001PCR<\/strong>\u5b9e\u9a8c\u5ba4\u5e73\u9762\u5e03\u5c40<\/strong><\/p>\n

PCR\u6269\u589e\u68c0\u9a8c\u5b9e\u9a8c\u5ba4\u539f\u5219\u4e0a\u5206\u4e3a\u56db\u4e2a\u5355\u72ec\u7684\u5de5\u4f5c\u533a\u57df\uff1a\u8bd5\u5242\u8d2e\u5b58\u548c\u51c6\u5907\u533a\u3001\u6807\u672c\u5236\u5907\u533a\u3001\u6269\u589e\u53cd\u5e94\u6df7\u5408\u7269\u914d\u5236\u548c\u6269\u589e\u533a\u3001\u6269\u589e\u4ea7\u7269\u5206\u6790\u533a\u3002\u4e3a\u907f\u514d\u4ea4\u53c9\u6c61\u67d3\uff0c\u8fdb\u5165\u5404\u4e2a\u5de5\u4f5c\u533a\u57df\u5fc5\u987b\u4e25\u683c\u9075\u5faa\u5355\u4e00\u65b9\u5411\u8fdb\u884c\uff0c\u5373\u53ea\u80fd\u4ece\u8bd5\u5242\u8d2e\u5b58\u548c\u51c6\u5907\u533a\u2192\u6807\u672c\u5236\u5907\u533a\u2192\u6269\u589e\u53cd\u5e94\u6df7\u5408\u7269\u914d\u5236\u548c\u6269\u589e\u533a\u2192\u6269\u589e\u4ea7\u7269\u5206\u6790\u533a\u3002 \u5404\u5b9e\u9a8c\u533a\u4e4b\u95f4\u7684\u8bd5\u5242\u53ca\u6837\u54c1\u4f20\u9012\u5e94\u901a\u8fc7\u4f20\u9012\u7a97\u8fdb\u884c\u3002<\/p>\n

\"PCR\u5b9e\u9a8c\u5ba4\u4f20\u9012\u7a97\u8bbe\u8ba1\u8981\u6c42\u3010\u89c4\u8303\u3011\"

PCR\u5b9e\u9a8c\u5ba4\u5e73\u9762\u5e03\u5c40 <\/p><\/div>\n

2\u3001<\/strong>\u5b9e\u9a8c\u5ba4\u7a7a\u8c03\u901a\u98ce\u7cfb\u7edf\u8bbe\u8ba1\u53ca\u538b\u529b\u63a7\u5236<\/strong><\/p>\n

PCR\u5b9e\u9a8c\u5ba4\u5e76\u6ca1\u6709\u4e25\u683c\u7684\u51c0\u5316\u8981\u6c42\uff0c\u4f46\u662f\u4e3a\u907f\u514d\u5404\u4e2a\u5b9e\u9a8c\u533a\u57df\u95f4\u4ea4\u53c9\u6c61\u67d3\u7684\u53ef\u80fd\u6027\uff0c\u5b9c\u91c7\u7528\u5168\u9001\u5168\u6392\u7684\u6c14\u6d41\u7ec4\u7ec7\u5f62\u5f0f\u3002\u540c\u65f6\uff0c\u8981\u4e25\u683c\u63a7\u5236\u9001\u3001\u6392\u98ce\u7684\u6bd4\u4f8b\u4ee5\u4fdd\u8bc1\u5404\u5b9e\u9a8c\u533a\u7684\u538b\u529b\u8981\u6c42\u3002<\/p>\n

2.1\u3001<\/strong>\u8bd5\u5242\u8d2e\u5b58\u548c\u51c6\u5907\u533a<\/strong><\/p>\n

\u8be5\u5b9e\u9a8c\u533a\u4e3b\u8981\u8fdb\u884c\u7684\u64cd\u4f5c\u4e3a\u8d2e\u5b58\u8bd5\u5242\u7684\u5236\u5907\u3001\u8bd5\u5242\u7684\u5206\u88c5\u548c\u4e3b\u53cd\u5e94\u6df7\u5408\u6db2\u7684\u5236\u5907\u3002\u8bd5\u5242\u548c\u7528\u4e8e\u6837\u54c1\u5236\u4f5c\u7684\u6750\u6599\u5e94\u76f4\u63a5\u8fd0\u9001\u81f3\u8be5\u533a\uff0c\u4e0d\u5f97\u7ecf\u8fc7\u5176\u4ed6\u533a\u57df\u3002\u8bd5\u5242\u539f\u6750\u6599\u5fc5\u987b\u8d2e\u5b58\u5728\u672c\u533a\u5185\uff0c\u5e76\u5728\u672c\u533a\u5185\u5236\u5907\u6210\u6240\u9700\u7684\u8d2e\u5b58\u8bd5\u5242\u3002 \u5bf9\u4e0e\u6c14\u6d41\u538b\u529b\u7684\u63a7\u5236\uff0c\u672c\u533a\u5e76\u6ca1\u6709\u4e25\u683c\u7684\u8981\u6c42\u3002<\/p>\n

\"PCR\u5b9e\u9a8c\u5ba4\u4f20\u9012\u7a97\u8bbe\u8ba1\u8981\u6c42\u3010\u89c4\u8303\u3011\"<\/p>\n

2.2\u3001<\/strong>\u6807\u672c\u5236\u5907\u533a<\/strong><\/p>\n

\u8be5\u533a\u57df\u4e3b\u8981\u8fdb\u884c\u7684\u64cd\u4f5c\u4e3a\u6837\u672c\u7684\u4fdd\u5b58\u3001\u6838\u9178(RNA\u3001DNA)\u63d0\u53d6\u3001\u8d2e\u5b58\u53ca\u5176\u52a0\u5165\u81f3\u6269\u589e\u53cd\u5e94\u7ba1\u548c\u6d4b\u5b9aDNA\u7684\u5408\u6210\u3002 \u672c\u533a\u7684\u538b\u529b\u68af\u5ea6\u8981\u6c42\u4e3a\uff1a\u76f8\u5bf9\u4e8e\u90bb\u8fd1\u533a\u57df\u4e3a\u6b63\u538b\uff0c\u4ee5\u907f\u514d\u4ece\u90bb\u8fd1\u533a\u8fdb\u5165\u672c\u533a\u7684\u6c14\u6eb6\u80f6\u6c61\u67d3\u3002\u53e6\u5916\uff0c\u7531\u4e8e\u5728\u52a0\u6837\u64cd\u4f5c\u4e2d\u53ef\u80fd\u4f1a\u53d1\u751f\u6c14\u6eb6\u80f6\u6240\u81f4\u7684\u6c61\u67d3\uff0c\u6240\u4ee5\u5e94\u907f\u514d\u5728\u672c\u533a\u5185\u4e0d\u5fc5\u8981\u7684\u8d70\u52a8\u3002<\/p>\n

2.3\u3001<\/strong>\u6269\u589e\u53cd\u5e94\u6df7\u5408\u7269\u914d\u5236\u548c\u6269\u589e\u533a<\/strong><\/p>\n

\u8be5\u533a\u57df\u4e3b\u8981\u8fdb\u884c\u7684\u64cd\u4f5c\u4e3aDNA\u6269\u589e\u3002\u6b64\u5916\uff0c\u5df2\u5236\u5907\u7684DNA\u6a21\u677f (\u6765\u81ea\u6837\u672c\u5236\u5907\u533a)\u7684\u52a0\u5165\u548c\u4e3b\u53cd\u5e94\u6df7\u5408\u6db2(\u6765\u81ea\u8bd5\u5242\u8d2e\u5b58\u548c\u5236\u5907\u533a)\u5236\u5907\u6210\u53cd\u5e94\u6df7\u5408\u6db2\u7b49\u4e5f\u53ef\u5728\u672c\u533a\u5185\u8fdb\u884c\u3002 \u672c\u533a\u7684\u538b\u529b\u68af\u5ea6\u8981\u6c42\u4e3a\uff1a\u76f8\u5bf9\u4e8e\u90bb\u8fd1\u533a\u57df\u4e3a\u8d1f\u538b\uff0c\u4ee5\u907f\u514d\u6c14\u6eb6\u80f6\u4ece\u672c\u533a\u6f0f\u51fa\u3002\u4e3a\u907f\u514d\u6c14\u6eb6\u80f6\u6240\u81f4\u7684\u6c61\u67d3\uff0c\u5e94\u5c3d\u91cf\u51cf\u5c11\u5728\u672c\u533a\u5185\u7684\u4e0d\u5fc5\u8981\u7684\u8d70\u52a8\u3002\u4e2a\u522b\u64cd\u4f5c\u5982\u52a0\u6837\u7b49\u5e94\u5728\u8d85\u51c0\u53f0\u5185\u8fdb\u884c\u3002<\/p>\n

2.4\u3001<\/strong>\u6269\u589e\u4ea7\u7269\u5206\u6790\u533a<\/strong><\/p>\n

\u8be5\u533a\u57df\u4e3b\u8981\u8fdb\u884c\u7684\u64cd\u4f5c\u4e3a\u6269\u589e\u7247\u6bb5\u7684\u6d4b\u5b9a\u3002 \u672c\u533a\u662f\u6700\u4e3b\u8981\u7684\u6269\u589e\u4ea7\u7269\u6c61\u67d3\u6765\u6e90\uff0c\u56e0\u6b64\u5bf9\u672c\u533a\u7684\u538b\u529b\u68af\u5ea6\u7684\u8981\u6c42\u4e3a\uff1a\u76f8\u5bf9\u4e8e\u90bb\u8fd1\u533a\u57df\u4e3a\u8d1f\u538b\uff0c\u4ee5\u907f\u514d\u6269\u589e\u4ea7\u7269\u4ece\u672c\u533a\u6269\u6563\u81f3\u5176\u5b83\u533a\u57df\u3002<\/p>\n

3\u3001<\/strong>\u6c61\u67d3\u7684\u9884\u9632\u4e0e\u63a7\u5236<\/strong><\/p>\n

PCR\u5b9e\u9a8c\u5ba4\u8bbe\u8ba1\u7684\u6838\u5fc3\u95ee\u9898\u662f\u5982\u4f55\u907f\u514d\u6c61\u67d3\u3002\u5728\u5b9e\u9645\u5de5\u4f5c\u4e2d\uff0c\u5e38\u89c1\u7684\u6709\u4ee5\u4e0b\u51e0\u79cd\u6c61\u67d3\u7c7b\u578b\uff1a\u6269\u589e\u4ea7\u7269\u7684\u6c61\u67d3\uff1b\u5929\u7136\u57fa\u56e0\u7ec4DNA\u7684\u6c61\u67d3\uff1b\u8bd5\u5242\u7684\u6c61\u67d3\u4ee5\u53ca\u6807\u672c\u95f4\u7684\u6c61\u67d3\u3002\u7531\u4e8e\u4e00\u65e6\u53d1\u751f\u6c61\u67d3\uff0c\u5b9e\u9a8c\u5c31\u5fc5\u987b\u505c\u6b62\uff0c\u76f4\u5230\u627e\u5230\u6c61\u67d3\u6e90\u4e3a\u6b62\uff0c\u800c\u4e14\u5b9e\u9a8c\u7ed3\u679c\u5fc5\u987b\u4f5c\u5e9f\uff0c\u9700\u91cd\u65b0\u8fdb\u884c\u5b9e\u9a8c\u3002\u6240\u4ee5\u53d1\u751f\u6c61\u67d3\u540e\u518d\u56f4\u7ed5\u5b9e\u9a8c\u5ba4\u6765\u5bfb\u627e\u6c61\u67d3\u6e90\u4e0d\u4f46\u8017\u65f6\u800c\u4e14\u7e41\u7410\uff0c\u6d6a\u8d39\u4eba\u529b\u7269\u529b\u3002\u56e0\u6b64\u8981\u907f\u514d\u6c61\u67d3\uff0c\u9996\u5148\u5e94\u662f\u9884\u9632\uff0c\u800c\u4e0d\u662f\u6392\u9664\u3002<\/p>\n

3.1\u3001<\/strong>\u5de5\u4f5c\u533a\u57df\u7684\u4e25\u683c\u5212\u5206<\/strong><\/p>\n

\uff081\uff09\u5404\u4e2a\u5b9e\u9a8c\u533a\u57df\u8bbe\u7f6e\u5408\u7406\uff1b<\/p>\n

\uff082\uff09\u5404\u4e2a\u5b9e\u9a8c\u533a\u57df\u8981\u6709\u660e\u663e\u7684\u6807\u8bb0\uff08\u5982\u9192\u76ee\u7684\u95e8\u724c\u6216\u4e0d\u540c\u7684\u5730\u9762\u989c\u8272\u7b49\uff09\uff0c\u4ee5\u907f\u514d\u5404\u4e2a\u4e0d\u540c\u5b9e\u9a8c\u533a\u57df\u8bbe\u5907\u7269\u54c1\u3001\u8bd5\u5242\u7b49\u53d1\u751f\u6df7\u6dc6\u3002<\/p>\n

3.2\u3001<\/strong>\u5408\u7406\u7684\u7cfb\u7edf\u8bbe\u7f6e<\/strong><\/p>\n

\uff081\uff09\u5408\u7406\u7684\u7a7a\u8c03\u901a\u98ce\u7cfb\u7edf\u8bbe\u7f6e\uff0c\u5c3d\u91cf\u91c7\u7528\u5168\u9001\u5168\u6392\u7684\u7a7a\u8c03\u7cfb\u7edf\uff1b<\/p>\n

\uff082\uff09\u4e25\u683c\u7684\u6c14\u6d41\u538b\u529b\u63a7\u5236\uff0c\u4fdd\u8bc1\u4e0d\u540c\u7684\u5b9e\u9a8c\u533a\u5185\u4e0d\u540c\u7684\u538b\u529b\u8981\u6c42\u3002<\/p>\n

3.3\u3001 <\/strong>\u89c4\u8303\u7684\u64cd\u4f5c<\/strong><\/p>\n

\uff081\uff09\u6269\u589e\u68c0\u9a8c\u5b9e\u9a8c\u5ba4\u7684\u6280\u672f\u4eba\u5458\u5fc5\u987b\u8fdb\u884c\u4e0a\u5c97\u57f9\u8bad\uff0c\u7ecf\u57f9\u8bad\u5408\u683c\u540e\u624d\u80fd\u4ece\u4e8b\u4e34\u5e8a\u57fa\u56e0\u6269\u589e\u68c0\u9a8c\u7684\u5de5\u4f5c\uff1b<\/p>\n

\uff082\uff09\u5728\u5b9e\u9a8c\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\uff0c\u64cd\u4f5c\u8005\u5fc5\u987b\u6234\u624b\u5957\uff0c\u5e76\u7ecf\u5e38\u66f4\u6362\u3002\u6b64\u5916\uff0c\u64cd\u4f5c\u4e2d\u4f7f\u7528\u4e00\u6b21\u6027\u5e3d\u5b50\u4e5f\u662f\u4e00\u4e2a\u6709\u6548\u5730\u9632\u6b62\u6c61\u67d3\u7684\u63aa\u65bd\uff1b<\/p>\n

\uff083\uff09\u6e05\u6d01\u5de5\u4f5c\u53ca\u65f6\u3001\u6b63\u786e\u3002\u5b9e\u9a8c\u5de5\u4f5c\u7ed3\u675f\u540e\uff0c\u5fc5\u987b\u7acb\u5373\u5bf9\u672c\u533a\u8fdb\u884c\u6e05\u6d01\u3002\u9664\u5e38\u89c4\u7684\u6d88\u6bd2\u6db2\u4f53\u5bf9\u8868\u9762\u8fdb\u884c\u64e6\u62ed\u6d88\u6bd2\u6216\u7d2b\u5916\u7ebf\u706f\u7684\u7167\u5c04\u6d88\u6bd2\u5916\uff0c\u5bf9\u4e00\u4e9b\u5b9e\u9a8c\u8bbe\u5907\u8fd8\u5e94\u8fdb\u884c\u9ad8\u538b\u6d88\u6bd2\u5904\u7406\u3002<\/p>\n

3.4\u3001<\/strong>\u4e25\u683c\u7684\u7ba1\u7406<\/strong><\/p>\n

\uff081\uff09\u4e25\u683c\u63a7\u5236\u8fdb\u51fa\u5b9e\u9a8c\u5ba4\u7684\u4eba\u5458\u3002\u4e0e\u5b9e\u9a8c\u65e0\u5173\u7684\u4eba\u5458\u4e0d\u5f97\u968f\u610f\u8fdb\u51fa\u5b9e\u9a8c\u5ba4\uff0c\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u8981\u8bbe\u7f6e\u72ec\u7acb\u7684\u901a\u9053\u548c\u8fdb\u51fa\u6574\u4e2a\u5b9e\u9a8c\u533a\u7684\u95e8\uff1b<\/p>\n

\uff082\uff09\u5c3d\u91cf\u51cf\u5c11\u5728\u5b9e\u9a8c\u533a\u5185\u4e0d\u5fc5\u8981\u7684\u8d70\u52a8\u4ee5\u51cf\u5c11\u4ea4\u53c9\u6c61\u67d3\u7684\u53ef\u80fd\u6027\u3002<\/p>\n

\uff083\uff09\u6269\u589e\u4ea7\u7269\u5206\u6790\u533a\u662f\u6700\u4e3b\u8981\u7684\u6269\u589e\u4ea7\u7269\u6c61\u67d3\u6765\u6e90\uff0c\u5e9f\u6db2\u4e0d\u80fd\u5728\u5b9e\u9a8c\u5ba4\u4e2d\u503e\u5012\uff0c\u5fc5\u987b\u7ecf\u6d88\u6bd2\u6db2\u6d78\u6ce1\u6d88\u6bd2\u540e\u5728\u8fdc\u79bb\u5b9e\u9a8c\u5ba4\u7684\u5730\u65b9\u5f03\u6389\uff0c\u7528\u8fc7\u7684\u5438\u5934\u7b49\u4e00\u6b21\u6027\u6750\u6599\u4e5f\u5e94\u7ecf\u6d88\u6bd2\u6db2\u6d78\u6ce1\u6d88\u6bd2\u540e\u7edf\u4e00\u5904\u7406\uff0c\u5982\u711a\u70e7\u7b49\uff1b<\/p>\n

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